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. 2020 Jan 16;21(2):574. doi: 10.3390/ijms21020574

Figure 3.

Figure 3

GRK2 mediates the βAR-induced enhancement of AngII-dependent aldosterone synthesis in AZG cells. H295R cells were treated for 2 h with 100 nM AngII alone or with 100 nM AngII and 10 µM isoproterenol (Iso) combined, in the presence of 30 µM Cmpd101 or after transfection with a CRIPSR lentivirus to knockout GRK5 (GRK5KO) or both (Cmpd101 + GRK5KO). At the end of the 2-h-long treatment, cells were harvested and total RNA was isolated to perform real-time PCR for StAR (A) or CYP11B2 (B) mRNA levels quantitation. Results were normalized based on the mRNA levels of the housekeeping gene 18S and are expressed as fold of control (no stimulation). (A) n = 5 independent experiments/group; (B) *, p < 0.05, vs. Control (No stimulation); NS: Not significant at p = 0.05; n = 5 independent experiments/group.