Figure 3.

DAB and NBT staining of silenced plants and the phenotypes of the detached leaves of silenced plants. (A) Phenotypes of the detached leaves of CaSBP12-silenced and control plants after 2 days of treatment with 400 mM NaCl. (B) DAB staining in leaves of CaSBP12-silenced and control plants after 2 days of treatment with 400 mM NaCl. (C) NBT staining in leaves of CaSBP12-silenced and control plants after 2 days of treatment with 400 mM NaCl. (D) Water-stained area (%) of the detached leaves of silenced plants after 2 days of treatment with 400 mM NaCl. (E) The DAB stained area (%) of the detached leaves of silenced plants after 2 days of treatment with 400 mM NaCl. (F) The NBT stained area (%) of the detached leaves of silenced plants after 2 days of treatment with 400 mM NaCl. (G) The H₂O₂ content of the detached leaves of silenced plants after 2 days of treatment with 400 mM NaCl. d: days. The black line is used as a scale bar (length 0.75 cm). Bars with different letters indicate significant differences at p ≤ 0.05. Mean values and SDs at least for three biological replicates are shown.