Table 1.

List of proteins altered at all three stages (2-day old, 4-day old without flooding, 4-day old with flooding) of soybean irradiated with millimeter waves.

Number	Accession a	Description	Functional Category b	Starting Point	Fold Change c	Flooded
					Unflooded
1	I1JGU8	phospho-2-dehydro-3-deoxyheptonate aldolase	amino acid metabolism	1.22	−0.61	−0.63
2	I1JEV7	carboxylesterase 2	biodegradation of xenobiotics	0.72	0.35	0.56
3	C6SX10	mitochondrial fission 1 protein	cell	−2.26	−1.39	−1.66
4	Q9M7N4	MFP1 attachment factor 1	cell	−1.51	−2.43	2.60
5	I1N3E1	phosphorylethanolamine cytidylyltransferase 1	cell	−0.72	2.31	−2.30
6	I1K6P4	uncharacterized protein	cell	1.02	−0.66	−1.58
7	Q09WE7	UDP-sugar pyrophosphorylase 1	cell wall	−1.22	0.81	0.38
8	V6CKR0	expansin	cell wall	−0.76	−0.38	0.54
9	Q9SEK9	seed maturation protein PM25	development	−1.75	−2.49	−1.06
10	I1L849	seed maturation protein	development	−0.88	−0.72	−2.80
11	I1LE33	cupin family protein	development	−1.89	−1.83	−2.36
12	I1L860	cupin family protein	development	−3.01	−3.54	−3.28
13	I1KY39	citrate synthase	gluconeogenese/glyoxylate cycle	−0.46	−0.40	0.56
14	I1LY51	12-oxophytodienoate reductase	hormone metabolism	0.70	0.53	0.37
15	A0A0R0IYE6	lipoxygenase	hormone metabolism	0.85	1.43	1.34
16	A0A0R0K553	uncharacterized protein	hormone metabolism	−1.08	−0.67	−0.38
17	A0A0R0GAV8	acyl-CoA dehydrogenase family member 10	lipid metabolism	−1.23	−0.54	0.88
18	I1MRK1	3-hydroxybutyryl-CoA epimerase	lipid metabolism	0.97	0.77	−2.38
19	I1M928	acyl-lipid (9-3)-desaturase isoform B	lipid metabolism	−2.52	1.61	0.98
20	O22378	metallothionein-II protein	metal handling	−3.07	2.56	0.40
21	I1K146	cytochrome c1-1, heme protein	mitoETC	−0.80	−0.60	0.45
22	C6SWW6	cytochrome c oxidase subunit 6a	mitoETC	1.11	−0.35	−0.98
23	I1KEH3	electron transfer flavoprotein subunit alpha	mitoETC	−0.38	−0.32	0.88
24	I1J9Q7	glutamate dehydrogenase	nitrogen metabolism	−1.31	−0.61	3.52
25	I1ML46	uncharacterized protein	nucleotide metabolism	1.53	1.08	1.06
26	Q2PMU2	photosystem I P700 chlorophyll a apoprotein A2	photosynthesis	1.52	0.70	3.04
27	P49161	cytochrome f	photosynthesis	1.21	0.85	0.69
28	A0A0R0I8Z5	chlorophyll a-b binding protein	photosynthesis	1.18	0.91	1.72
29	Q2PMQ9	photosystem II CP47 reaction center protein	photosynthesis	1.12	0.74	1.42
30	A0A0R4J389	photosystem I reaction center subunit III	photosynthesis	1.13	0.94	3.00
31	I1MUQ0	ATP synthase delta chain	photosynthesis	0.73	0.69	1.07
32	K7KJ72	uncharacterized protein	protein. degradation	0.24	−0.38	−0.51
33	I1M4G0	carboxypeptidase	protein. degradation	1.16	0.52	0.81
34	I1MIC1	metacaspase-4	protein. degradation	1.19	−0.29	0.30
35	C6TFY7	chaperonin 10	protein. folding	5.47	−0.63	−6.07
36	I1K5E6	guanine nucleotide-binding protein subunit	protein. post translational modification	0.38	−0.30	−0.48
37	C6SVV1	60S ribosomal protein	protein. synthesis	−1.37	−0.40	1.35
38	C6SXD3	40S ribosomal protein S24	protein. synthesis	0.51	−0.52	−0.95
39	I1MDJ2	60S ribosomal protein L23A isoform	protein. synthesis	0.90	−0.67	−1.05
40	A0A0R0EIR6	glycosyltransferase	protein. synthesis	2.63	2.39	−1.93
41	I1N1W7	mitochondrial-processing peptidase subunit	protein. targeting	−0.41	−0.41	0.40
42	I1L0S5	outer envelope pore protein 16-2	protein. targeting	−0.93	−0.91	1.39
43	C6TB70	1-cys peroxiredoxin	redox	−0.36	−0.69	−0.41
44	I1KFE9	L-ascorbate oxidase	redox	0.62	1.10	0.42
45	I1LCG9	pre-mRNA-processing factor 17	RNA	−0.24	−1.45	0.86
46	K7LRU6	apoptotic chromatin condensation inducer	RNA	−0.79	−0.61	0.72
47	I1KEW2	O-methyltransferase 3	secondary metabolism	0.54	0.38	0.59
48	F8WRI3	gamma-tocopherol methyltransferase	secondary metabolism	1.06	1.37	−2.06
49	I1K711	uncharacterized protein	signalling	0.48	0.54	0.72
50	I1LBC8	mitochondrial Rho GTPase	signalling	−1.08	−1.10	0.93
51	K7KIL0	GTP-binding nuclear protein	signalling	1.30	−0.30	−0.80
52	K7LDT9	low-temperature-induced 65 kDa protein	stress	−1.86	−1.96	−1.01
53	C6T0C7	trypsin inhibitor A	stress	0.88	1.11	1.20
54	A0A0R4J4G9	heat shock 22 kDa protein	stress	−1.19	−2.53	−1.19
55	A0A0R4J4S6	NADPH-protochlorophyllide oxidoreductase	tetrapyrrole synthesis	0.60	−0.57	0.90
56	C6TDZ1	aquaporin PIP2-7	transport	−0.68	1.25	0.92
57	I1JMI9	malic enzyme	tricarboxylic acid cycle	0.55	0.20	−0.38
58	P15490	stem 28 kDa glycoprotein	miscellaneous	1.55	0.92	1.54
59	K7L6U4	uncharacterized protein	miscellaneous	0.74	0.56	0.82
60	P10743	stem 31 kDa glycoprotein	miscellaneous	1.59	0.77	0.85
61	I1KZI4	2,3-dimethylmalate lyase	not assigned	−0.87	0.98	1.17
62	I1MRP7	uncharacterized protein	not assigned	−0.67	−0.66	1.51
63	K7LKS0	exocyst complex component SEC5	not assigned	−0.49	−1.08	−1.26
64	I1LV40	uncharacterized protein	not assigned	−0.46	−0.76	−0.70
65	I1NA37	uncharacterized protein	not assigned	−1.11	−1.91	0.86
66	K7MGN4	trafficking protein particle complex subunit	not assigned	0.57	0.66	−0.49

^a “accession” is determined according to UniprotKB Glycine max (Soybean) protein database. ^b “functional category” is obtained using MapMan bin codes. Abbreviations: cell, cell division/organization; mitoETC, mitochondrial electron transport chain; protein, protein synthesis/targeting/others; RNA, RNA processing/regulation of transcription. “not assigned” indicates protein without ontology or characterized functions. ^c “fold change” indicates log₂ fold change of identified proteins from millimeter waves irradiated soybean compared to control.