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. 2020 Jan 19;21(2):666. doi: 10.3390/ijms21020666

Figure 5.

Figure 5

Immunohistochemical and biochemical analysis of the deposition and solubility of the binding partners of matrilins. Immunohistochemistry at newborn (A) and 4 weeks (B) stages indicates no apparent differences in the deposition of collagen II (COL II), collagen VI (COL VI), collagen IX (COL IX), aggrecan (ACAN) and cartilage oligomeric matrix protein (COMP) between control and Matn1-4−/− mice. Original magnifications: × 10 for (A) and (B); x 20 for inserts in (A). Western blot analysis of cartilage extracts in newborn (C) and 4 weeks old (D) animals. In newborn, slightly increased extractability of collagen II, collagen IX, aggrecan, collagen VI and COMP in buffer I and significantly weaker signals for collagen II, collagen IX and COMP were detected in fraction III isolated from the cartilage of quadruple knockout mice compared with controls (C). At 4 weeks, the amount of aggrecan is increased in all fractions, the amount of collagen IX is slightly increased in fraction II and the amount of COMP is moderately decreased in fraction III in M Matn1-4−/− mice compared with the control (D). Abbreviation: MW-molecular weight marker.