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. 2019 Aug 12;102(1):84–91. doi: 10.1093/biolre/ioz158

Figure 2.

Figure 2

Phenotypic analysis of Prss44 knockout male mice. (A) Genomic structure and knockout strategy of mouse Prss44. Dual sgRNAs were designed to target the first and last coding exons. F1/F2, forward primer for genotyping; R1/R2, reverse primers for genotyping. (B) Genotype validation of Prss44 knockout mice by Sanger sequencing. (C) Genotype validation of Prss44 knockout mice by genomic PCR. (D) Comparison of testis weights of Prss44+/+ wildtype (WT) and Prss44−/− knockout (KO) mice. (E) Histological analyses of testes and epididymides in Prss44 WT and KO mice. (F) Morphology of spermatozoa extracted from the cauda epididymides of Prss44 WT and KO mice. Scale bars as indicated.