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. 2019 Aug 12;102(1):84–91. doi: 10.1093/biolre/ioz158

Figure 3.

Figure 3

Phenotypic analysis of Prss46 knockout male mice. (A) Genomic structure and knockout strategy of mouse Prss46. Dual sgRNAs were designed to target the first and last coding exons. F1/F2, forward primer for genotyping; R1/R2, reverse primers for genotyping. (B) Genotype validation of Prss46 knockout mice by Sanger sequencing. (C) Genotype validation of Prss46 knockout mice by genomic PCR. (D) Comparison of testis weights of Prss46+/+ wildtype (WT) and Prss46−/− knockout (KO) mice. (E) Histological analyses of testes and epididymides in Prss46 WT and KO mice. (F) Morphology of spermatozoa extracted from the cauda epididymides of Prss46 WT and KO mice. Scale bars as indicated.