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. 2019 Aug 12;102(1):84–91. doi: 10.1093/biolre/ioz158

Figure 4.

Figure 4

Phenotypic analysis of Prss54 knockout male mice. (A) Genomic structure and knockout strategy of mouse Prss54. Since sgRNAs was designed to target the first coding exons. F1/F2, forward primer for genotyping; R1/R2, reverse primers for genotyping. (B) Genotype validation of Prss54 knockout mice by Sanger sequencing. (C) Genotype validation of Prss54 knockout mice by genomic PCR. (D) Comparison of testis weights of Prss54+/+ wildtype (WT) and Prss54−/− knockout (KO) mice. (E) Histological analyses of testes and epididymides in Prss54 WT and KO mice. (F) Morphology of spermatozoa extracted from the cauda epididymides of Prss54 WT and KO mice. Scale bars as indicated.