Table 3.

Oligonucleotides used in the study.

Primer	Sequence	Target Gene	Target Bacterial Genus	PCR Cycling	Product Length	Reference
27F 1492R	5′-AGAGTTTGATCCTGGCTCAG-3′ 5′-GGTTACCTTGTTACGACTT-3′	16S rDNA	All tested	3 min 95 °C, 30 × (30 s 94 °C, 45 s 55 °C, 90 s 72 °C), 5 min 72 °C	1500 bp	[21]
PsEG30F PsEG790R	5′- ATYGAA ATCGCCAARCG-3′ 5′-CGGTTGATKT CCTTGA-3′	rpoD	Pseudomonas	3 min 95 °C, 30 × (60 s 94 °C, 45 s 55 °C, 50 s 72 °C), 5 min 72 °C	750 bp	[22]
Vic3 Vic2	5′-GGCGAAATGGCWGAGAACCA-3′ 5′-GAGTCTTCGAAGTTGTAACC-3′	rpoB	Citrobacter	4 min 94 °C, 30 × (30 s 94 °C, 30 s 50 °C, 45 s 72 °C), 5 min 72 °C	410 bp	[23]
Ac696F Ac1093R	5′-TAYCGYAAAGAYTTGAAAGAAG-3′ 5′-CMACACCYTTGTTMCCRTGA-3′	rpoB	Acinetobacter	3 min 95 °C, 30 × (60 s 94 °C, 52 s 45 °C, 60 s 72 °C), 5 min 72 °C	370 bp	[24]
359f 359r	5′-TTATCGCTCAGGCGAACTCCAAC-3′ 5′-TGCTGGATTCGCCTTTGCTACG-3′	rpoB	Serratia	3 min 95 °C, 30 × (50 s 94 °C, 40 s 52 °C, 60s 72 °C), 5 min 72 °C	530 bp	[25]
ESchr-rpoF ESchr-rpoR	5′GGTGAAGTAGTTTCTATCGAAAGA-3′ 5′-ATGTTTGGTCCTTCCGGAGTT-3′	rpoB	Chryseobacterium	3 min 95 °C, 30 × (35 s 95 °C, 35 s 52 °C, 50 s 72 °C), 5 min 72 °C	790 bp	This work
recAF recAR	5′-TCSGGYAARACCACSCTGAC-3′ 5′-RTACCAGGCRCCGGACTTCT-3′	recA	Pseudomonas	4 min 94 °C, 30 × (30 s 94 °C, 30 s 55 °C, 40 s 72 °C), 5 min 72 °C	600 bp	[26]