Table 4.
Molecular identification of bacterial strains with antimicrobial activity against EPB isolated from the midgut of M. melolontha larvae.
| Strain ID | Identification Result/Gene Accession Numbers | Strain with the Highest Similarity to the Isolate in the Gene Bank Based on the Gene Sequence/Gene Accession Number/% Nucleotide Identity | ||
|---|---|---|---|---|
| 16S rDNA | rpoD/rpoB | recA | ||
|
MT3
L2 05 |
Pseudomonas chlororaphis 16S rDNA—MM421924 rpoD—MN445046 |
P. chlororaphis subsp. piscum JF3835T (FJ168539)—99.8% | * P. chlororaphis subsp. piscum ZJU60 (CP027656)—98.2%; P. chlororaphis subsp. aurefaciens NCIMB 9265 (AB039555)—98.2% |
n.d. |
|
MT3
L2 08 |
Pseudomonas chlororaphis 16S rDNA—MM421925 rpoD—MN445047 |
P. chlororaphis subsp. piscum JF3835T (FJ168539)—99.8% | * P. chlororaphis subsp. piscum ZJU60 (CP027656)—98.2%; P. chlororaphis subsp. aurefaciens NCIMB 9265 (AB039555)—98.2% |
n.d. |
|
MT3
L2 10 |
Pseudomonas chlororaphis 16S rDNA—MM421926 rpoD—MN445048 recA—MN477250 |
P. chlororaphis subsp. piscum JF3835T (FJ168539)—99.8% | * P. chlororaphis subsp. piscum ZJU60 (CP027656)—98.0%; P. chlororaphis subsp. aurefaciens NCIMB 9265 (AB039555)—98.0% |
P. chlororaphis subsp. piscum JF3835T (FJ168540)—98.4% |
|
MT3
L2 13 |
Pseudomonas chlororaphis 16S rDNA—MM421923 rpoD—MN445049 |
P. chlororaphis subsp. piscum JF3835T (FJ168539)—99.9% | * P. chlororaphis subsp. piscum ZJU60 (CP027656)—98.0%; P. chlororaphis subsp. aurefaciens NCIMB 9265 (AB039555)—98.0% |
n.d. |
|
MTA1
L2 01 |
Citrobacter murlinae 16S rDNA—MM421947 rpoB—MN445055 |
C. murliniae CIP 104556T (KY178281)—99.3% | ** C. murliniae CIP 104556T (KM516007)—99.5% | n.d. |
|
MMC3
L3 04 |
Pseudomonas chlororaphis 16S rDNA—MM421927 rpoD—MN445050 recA—MN477251 |
P. chlororaphis subsp. piscum JF3835T (FJ168539)—99.8% | * P. chlororaphis subsp. piscum ZJU60 (CP027656)—98.2%; P. chlororaphis subsp. aurefaciens NCIMB 9265 (AB039555)—98.2% |
P. chlororaphis subsp. piscum JF3835T (FJ168540)—98.6% |
|
MTB1
L3 12 |
Serratia liquefaciens 16S rDNA—MM422010 rpoB—MN445052 |
Serratia quinivorans DSM4597T (AJ233435)—99.4%; S. liquefaciens CIP 103238T (NR 042062)—98.9% |
** S. liquefaciens LMG7884T (JX425335)—99.4% S. quinivorans LMG7887T (JX425309)—97.7% |
n.d. |
|
MTA3
L2 13 |
Acinetobacter calcoaceticus 16S rDNA—MN429316 rpoB—MN445056 |
A. calcoaceticus DSM 30006T (AJ633632)—99.8% | ** A. calcoaceticus CIP 81.8T (DQ207474)—97.9%; A. calcoaceticus CA16 (NZ_CP020000)—98.7% |
n.d. |
| MTS3 L3 15 |
Pseudomonas chlororaphis 16S rDNA—MM421928 rpoD—MN445051 recA—MN477252 |
P. chlororaphis subsp. piscum JF3835T (FJ168539)—99.7% | * P. chlororaphis subsp. piscum ZJU60 (CP027656)—98.4%; P. chlororaphis subsp. aurefaciens NCIMB 9265—98.4% |
P. chlororaphis subsp. piscum JF3835T (FJ168540)—98.6% |
|
MTC1
L3 03 |
Serratia sp. 16S rDNA—MM422009 rpoB—MN445053 |
Serratia grimesii DSM 30063T (AJ233430)—99.3%; Serratia proteamaculans DSM 4543T (AJ233434)—99.2% S. liquefaciens CIP 103238 (AJ306725)—99.1% |
** S. liquefaciens LMG7884T (JX425335)—98.8% S. quinivorans LMG7887T (JX425309)—98.3% |
n.d. |
|
MMH3
L2 04 |
Chryseobacterium lathyri 16S rDNA—MM429317 rpoB—MN445063 |
C. lathyri RBA2-6T (DQ673674)—99.8% | ** C. lathyri KCTCT 22544T (NZ_QNFY01000004)—99.1% | n.d. |
|
MMH4
L2 06 |
Chryseobacterium sp. 16S rDNA—MN429318 rpoB—MN445064 |
C. nakagawai NCTC 13529T (NZLR134386)—98.7% | ** C. joostei DSM 16927T (CP033926)—93.6%; C. nakagawai NCTC 13529TT (LR134386)—91.0% |
n.d. |
n.d.—not determined, * rpoD gene sequences analysis; ** rpoB gene sequences analysis.