Table 3.
Method validation results including linearity, LOD, LOQ, stability, precision and repeatability.
| No. | Name | Linearity | LOD (ng/mL) | LOQ (ng/mL) | Stability (RSD, %) | Precision (RSD, %) | Repeatability (RSD, %) | ||
|---|---|---|---|---|---|---|---|---|---|
| Regression Equations | R2 | Ranges (ng/mL) | |||||||
| 1 | nobiletin | y = 62,293,800x − 1,576,600 | 0.9968 | 7.81–1000 | 0.488 | 0.977 | 3.05 | 1.43 | 4.44 |
| 2 | narcissin | y = 3,029,650x − 314,384 | 0.9942 | 31.25–4000 | 7.81 | 15.63 | 3.26 | 3.22 | 2.26 |
| 3 | isorhamnetin-3-O- beta-d-glucoside |
y = 279,387x − 11,500 | 0.9973 | 62.5–4000 | 15.63 | 31.25 | 4.22 | 4.37 | 4.93 |
| 4 | tangeretin | y = 4,034,570x − 18,086 | 0.9902 | 3.91–250 | 0.488 | 0.977 | 1.94 | 1.76 | 4.87 |
| 5 | rutin | y = 3,089,740x − 61,316 | 0.9978 | 15.63–1000 | 7.81 | 31.25 | 2.99 | 3.71 | 1.74 |
| 6 | quercetin | y = 17,556,400x − 169,184 | 0.9921 | 15.63–500 | 7.81 | 15.63 | 3.82 | 4.03 | 4.9 |
| 7 | isorhamnetin | y = 116,434,300x − 149,754 | 0.9903 | 7.81–250 | 3.91 | 7.81 | 2.53 | 2.94 | 2.82 |
| 8 | quercetin-7-O- glucoside |
y = 5,857,970x − 144,514 | 0.9989 | 31.25–1000 | 7.81 | 15.63 | 3.26 | 3.31 | 2.39 |
| 9 | kaempferol-3-O- glucoside |
y = 4,499,260x − 117,172 | 0.9901 | 31.25–1000 | 7.81 | 15.63 | 2.06 | 4.49 | 4.9 |