Table 1.
Antibody | Company | Dilution | Product No. |
---|---|---|---|
VE-cadherin | Abgent | 1:1000 | AP27724a |
Twist | Abcam | 1:500 | ab5088 |
β-actin | Sigma-Aldrich | 1:20,000 | A5316 |
N-cadherin | CST | 1:1000 | 4061 |
Vimentin | CST | 1:1000 | 5741 |
Phospho-AKT | CST | 1:1000 | 4060 |
AKT | CST | 1:2000 | 4691 |
goat anti-rabbit IgG-HRP | CST | 1:5000 | 7074P2 |
goat anti-mouse IgG-HRP | Bio-Rad | 1:5000 | STAR120P |
Abgent (San Diego, CA, USA); Abcam (Cambridge, UK); Sigma-Aldrich (St. Louis, MO, USA); CST, Cell Signaling Technology (Beverly, MA, USA); Bio-Rad (Langford Lane, Kidlington).