Figure 3.

Alignment of amino acid sequence and subcellular localization of GmIREG3. (A) Amino acid sequence alignment of IREG proteins from soybean (GmIREG3), buckwheat (FeIREG1) and Arabidopsis (AtIREG1 and AtIREG2). (B) Subcellular localization of a C-terminal GmIREG3-GFP fusion protein in Nicotiana benthamiana and the fusion protein was driven by 35S promoter. At 3 d after infiltration, the fluorescence signals were visualized by confocal microscopy. GmIREG3-GFP was colocated with the tonoplast marker to the tonoplast. Red rectangles indicate the tonoplast separated from the plasma membrane and white arrows indicate the plasma membrane.