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Crocetin reduces the levels of mesenchymal markers and induces an increase in neuronal ones in glioma cells, which could be related to histone deacetylase (HDAC) expression. (A) Representative Fluorescence-Activated Cell Sorter (FACS) histograms and (B) Western blotting analyses of HDAC1 and HDAC3 levels. Analyses was made at 72 h in cells after treatment with 250 and 500 μM of crocetin. Cell extract samples were loaded with 20 μg of protein per lane.
