Figure 3.

CCT inhibits the expression of FASN and CD44 proteins, thereby inducing cell apoptosis and reducing migratory capacity. (A) Western blot analyses of whole protein extracts harvested from treated (250 and 500 μM CCT) and untreated GBM cell lines for CD44, FASN, and activated caspase 3, for normalized versus GAPDH. Lanes were loaded with 20 μg of protein. (B) The wound-healing repair assay was performed on the U87MG and U251 cells. The images were acquired at T0 and T24 h. Quantization was performed by Image J software, as indicated in panel B.