Figure 6.

Crocetin increased disease-free and overall survival in orthotopic intra-brain tumors as determined by using a differentiated Luc-U251MG cell model. Luciferase-tagged U251 cells (Luc-U251) were orthotopically injected into the brains of CD1 mice, as described. After 5 days (when no tumors were organized into the brain) the animals were randomized and treated with CCT (100 mg/kg/day per os), temozolomide (16 mg/kg for 5 consecutive days), RT (single dose of 4 Gy administered at day 5), and a combination of RT + TMZ (standard of care). (A) Schema of treatments and performed analyses. (B) Disease-free survival graph representation. (C) Overall survival data: graphical representation. (D) Kaplan–Meier graphical analyses.