Table 1.

Kinetic parameters of PhNah20A and β-NAHAs from the literature on pNPGlcNAc and pNPGalNAc. Sm—S. marcescens; Ah—A. hydrophila; Bb—B. bifidum; Cf—C. fimi; Vf—V. furnissii; Eh—E. histolytica; Tr—Trichoderma reesei.

Enzyme	Substrate	KM (mM)	Vmax (µmoL·mg−1·min−1)	kcat (s−1)	kcat/KM (mM−1·s−1)
PhNah20A	pNPGlcNAc	0.43 ± 0.07	93.7 ± 5.0	146.8	341
	pNPGalNAc	0.56 ± 0.11	123.0 ± 7.0	192.7	344
SmChb 1	pNPGlcNAc	56.7 ± 4.3	NI	111.0	1.95
AhNag20A 2	pNPGlcNAc	0.52	115	NI	NI
	pNPGalNAc	0.5	7.6	NI	NI
AhNagB 2	pNPGlcNAc	8.57	25	NI	NI
	pNPGalNAc	11.1	11	NI	NI
BbhI of Bb 3	pNPGlcNAc	120.0 ± 0.2	NI	213	178
	pNPGalNAc	NA	NA	NA	NA
CfHex20 4	pNPGlcNAc	0.053	NI	482.3	9090
	pNPGalNAc	0.066	NI	129.1	1950
VfExoI 5	pNPGlcNAc	0.09	270	NI	NI
	pNPGalNAc	0.33	130	NI	NI
Hex2 6	pNPGlcNAc	0.48	NI	60.0 ± 1.7	NI
EhHexA 7	pNPGlcNAc	0.1	3.8	NI	NI
TrNag1	pNPGlcNAc	69.4 ± 4.0	NI	NI	1023 ± 23

Data from ¹ [69], ² [36] and [34], ³ [40], ⁴ [39], ⁵ [33], ⁶ [18], ⁷ [67]. NI—not indicated; NA—no detected activity.