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. 2020 Jan 14;21(2):513. doi: 10.3390/ijms21020513

Figure 5.

Figure 5

Substitution rate analysis of the impact of st8sia gene duplications and losses. The sialylmotifs are indicated by red boxes and the transmembrane domain by a grey box. (A) Duplication of st8sia2 genes in Salmoniformes. The substitutions observed in ST8Sia II-r1 and ST8Sia II-r2 are indicated by an arrow above and below, respectively. The position of the substitutions corresponds to the alignment in Supplemental Data 2. The black rectangles correspond to convergent mutations retrieved in both sequences. In T > K, for example, T is the ancestral state and K is the derived one. (B) Impact of st8sia4 gene loss in Neoteleostei on the remaining fish ST8Sia II sequences. The code for substitution is the same as in A. The corresponding amino acid present in the paralogue ST8Sia IV sequence is given below. (C) Impact of st8sia9 gene loss in Otocephala on the remaining fish ST8Sia III sequences (same abbreviations as in B).