Figure 1.

RG-like Cells in Human Adult GBMs

(A) Representative confocal images of GBM sections. Cells in M phase are labeled with phospho-histone 3 (pH3) and their radial fiber-like processes are detected with pVIM.

(B) Percentage of pVIM+ and pH3+ cells in human GBMs (n = 21 tumors).

(C) Quantification of co-expression of pVim with an array of RG markers, by immunochemistry (IHC).

(D) Schematic flow of the development of 3D explants from patient GBM samples. IHC demonstrates the expression of pH3 and pVim during cell division; arrows points to mitotic cells with or without process (labeled RG-like or non-RG-like).

(E) Co-expression of RG-like markers in pVim+ cells in explants.

Scale bars, 10 and 5 μm (A), 20 and 10 μm (D and E).