Figure 4.

Inflammation Regulates Cell-Cycle States of RG-like Cells

(A) Immunohistochemistry for GLAST and protein synthesis (OP-puro) in growth factor (GF) and GF-free conditions.

(B) IL-1b treatment activates RG-like cells coexpressing GLAST⁺LIFR⁺ by stimulating protein synthesis (OP-puro) and the expression of EGFR. Quantification of general protein synthesis and expression of EGFR protein in the indicated cell groups (right panel), p = 0.0008 (OP-puro), p = 0.019 (EGFR).

(C) RNA-FISH showing that IL-1b activates the transcription of EGFR in RG-like cells and while CRYAB expression is maintained.

(D) Treatment with IL-1b for 72 h significantly increases Ki67 in GLAST⁺LIFR⁺ cells, signifying entry into the cell cycle (n = 6, triplicate from two patients). Scale bars, 20 μm (A, B, and D), 50 μm (C).

(E and F) IHC for GLAST (E) in a set of matching primary (left) and recurrent (right) tumors quantified in (F). n = 4 pairs, Mann-Whitney.