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. 2020 Jan 15;21(2):544. doi: 10.3390/ijms21020544

Table 2.

Forward (for) and reverse (rev) primer sequences of the reference gene polyubiquitine, vascular endothelial growth factor A (VEGFA) isoforms and VEGF receptors (VEGFR-1 and VEGFR-2) for bovine used in quantitative reverse transcription-PCR.

Primer Sequence Fragment Length [bp] Accession Number
Polyubiquitin for AGATCCAGGATAAGGAAGGCAT 198 NM_174133
rev GCTCCACCTCCAGGGTGAT
VEGFA-121 for CCGTCCCATTGAGACCCTG 208 AB455252
rev CGGCTTGTCACAATTTTTCTTGTC
VEGFA-165 for CCGTCCCATTGAGACCCTG 278 NM_174216
rev GCCCACAGGGATTTTCTTGC
VEGFA-189 for CCGTCCCATTGAGACCCTG 297 AB450824
rev TGCCCTTTGCCCTTTCCTC
VEGFR-1 for CACCAAGAGCGACGTGTG 351 X94263
rev AAGAAGTCCTCGGAGAAGGC
VEGFR-2 for AGACTGGTTCTGGCCCAAC 379 X94298
rev GAAGCCTTTCTGGCTGTC