Figure 3.

Selection and characterization of phage displayed anti-ZIKV_E single-chain fragment (scFv) library against ZIKV_E recombinant proteins. The biopanning procedure used constructed antibody libraries with short- and long-linkers. The eluted phage titers were determined after each cycle of panning for four rounds. (A) Number of eluted phages in terms of colony-forming units (cfu), (B) amplified phages tested against ZIKV_E F2 protein for each round of panning, (C) eluted phages obtained after panning against ZIKV_E F3 protein, and (D) amplified phages from each cycle of panning to test binding ability against ZIKV_E F3 protein. Anti-ZIKV_E IgY antibodies extracted from immunized chicken eggs were used as a positive control. The library phages were used as a reference point to the amplified phages of four round biopanning.