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. 2020 Jan 13;21(2):492. doi: 10.3390/ijms21020492

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sequence analysis, expression, purification, and characterization of four anti-ZIKV_E scFv antibodies. (A) Randomly selected scFv clones with long-linker against ZIKV_E F2 protein, (B) scFv clones with short-linker against ZIKV_E F3 proteins, (C) scFv clones with long-linker against ZIKV_E F3 protein. (D) The anti-ZIKV_E scFv antibodies with long- or short-linkers and their predicted amino acid sequences were compared with that of the chicken germ line. Framework region (FR) and complementary determining region (CDR) boundaries were indicated above the germ line gene sequences. Four representative clones were identified. (E) The scFv antibodies expressed in Escherichia coli (E. coli) were purified by nickel beads and analyzed on SDS-PAGE. (F) The identities of purified scFv antibodies were confirmed by western blot.

Sequence analysis, expression, purification, and characterization of four anti-ZIKV_E scFv antibodies. (A) Randomly selected scFv clones with long-linker against ZIKV_E F2 protein, (B) scFv clones with short-linker against ZIKV_E F3 proteins, (C) scFv clones with long-linker against ZIKV_E F3 protein. (D) The anti-ZIKV_E scFv antibodies with long- or short-linkers and their predicted amino acid sequences were compared with that of the chicken germ line. Framework region (FR) and complementary determining region (CDR) boundaries were indicated above the germ line gene sequences. Four representative clones were identified. (E) The scFv antibodies expressed in Escherichia coli (E. coli) were purified by nickel beads and analyzed on SDS-PAGE. (F) The identities of purified scFv antibodies were confirmed by western blot.