Table 1.

List of primers used for PCR amplification of Zika virus envelope (ZIKV_E) gene from pcDNA3.1_ZIKV E plasmid DNA.

Primer Name	Oligonucleotide (5′–3′)
ZIKVEFLF	TAAAATGGATCCATGATCAGGTGCATAGGAGTCAGC
ZIKVEFLR	AATTTTCTCGAGAGCAGAGACGGCTGTGGATAAG
ZIKVEF3F	AATTAAGGATCCGACATTCCATTACCTTGGCACGCT
ZIKVEF3R	TTAATTCTCGAGGATTACGGGGTTAGCGGTTATCAAC
ZIKVEF2R	TAAATTCTCGAGGGCATGTGCGTCCTTGAACTCTAC
ZIKVEF4F	TTATAAGGATCCACAGATGGACCTTGCAAGGTTCCA

Underlined nucleotides: digested sites for restriction endonucleases. ATG is underline to show that it was added to the original sequence. It could not be digested during PCR process. The start codon was added because the ZIKV envelope gene sequence has no ATG codon from the beginning of the sequence (Figure S2).