Histological change and serum biochemistry of HFD-fed mice. SREBP decoy ODN treatment effectively suppresses fatty change, serum aspartate aminotransferase (AST), alanine transaminase (ALT), total cholesterol, and triglycerides (n = 6). (A) H&E staining; Scale bar = 100 μm. (B) Fatty change levels; (C) Serum AST, ALT, total cholesterol, and triglycerides. Representative images from each group. (D) Western blot results show that cleaved caspase-3 expression in liver tissue. The expression levels of the protein from quantification of these images by Image J. NC, normal control group; SREBP ODN, group treated with SREBP decoy ODN; HFD + Scr ODN, HFD fed group treated with scrambled decoy ODN; HFD + SREBP ODN, HFD fed group treated with SREBP decoy ODN. * p < 0.05 compared to the NC group. † p < 0.05 compared to the SREBP ODN group. ‡ p < 0.05 compared to the HFD + Scr ODN group.