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. 2020 Jan 12;21(2):479. doi: 10.3390/ijms21020479

Figure 2.

Figure 2

DNA methylation analysis in the TNF-α promoter region. CpG site-specific DNA methylation analyses at the TNF-α promoter region in visceral adipose tissue from mothers with NGT vs. GDM. (A) Schematic illustration of the DNA methylation assays (R1, R2, and R3) for the TNF-α promoter region, including transcription factor binding sites (TFBS) Sp1, EGR1, AP2 (diamonds). (B) Percent DNA methylation at each individual CpG site investigated (10 CpG sites) in VAT of normal glucose tolerant (NGT; white; n = 22) vs. GDM group (GDM; black; n = 19). Overall mean across CpG sites is also included. Data are means ± S.E.M. * P < 0.05. TSS: Transcriptional start site, bp: basepairs.