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. 2020 Jan 16;21(2):593. doi: 10.3390/ijms21020593

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Representative microscopic fields of non-transfected and SV40 transfected ACL ligamentocytes on PLA scaffolds. Experimental design, cell expansion (A1), self-assembly using hanging drop method (50,000 cells per spheroid) (A2), macroscopic image of the wet PLA scaffolds (A3), and dynamical rotatory scaffold culture (A4). (B1–E2) Live/Dead staining of cultures up to day 35 (non-transfected) and day 28 (SV40 transfected) cells. (B1–E1) Non-transfected. (B2–E2) SV40 transfected ligamentocytes. Vital cells: Green. Dead cells: Red. Scaffold fibers: Slightly red colored. Three independent experiments were performed. Scale bars: 100 µm.