| 2D | two-dimensional |
| 3D | three-dimensional |
| AB | alcian blue |
| ACL | anterior cruciate ligament |
| DAPI | 4’,6-diamidino-2-phenylindole |
| DMEM | Dulbecco’s Modified Eagle’s |
| ECM | extracellular matrix |
| FCS | fetal calf serum |
| FDA | fluorescein diacetate |
| HE | hematoxylin eosin staining |
| HMDI | hexamethylene diisocyanate |
| HPRT | hypoxanthine-guanine phosphoribosyltransferase |
| hTERT | human telomerase reverse transcriptase |
| PBS | phosphate buffered saline |
| PFA | paraformaldehyde |
| PI | propidium iodide |
| RT | room temperature |
| SD | standard deviation |
| SV40 | Simian virus 40 |
| sGAGs | sulphated glycosaminoglycans |
| TBS | TRIS buffered saline |
| TE | tissue engineering |