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. 2020 Jan 17;21(2):610. doi: 10.3390/ijms21020610

Figure 1.

Figure 1

Beige and white adipocyte markers expression and their modulation by four differentiation-inducing cocktails in 3T3-L1 cells. (A) Relative expression of UCP1, TIMM44, NRG4, ADIPO, and LPL in 3T3-L1 fibroblasts that were induced to differentiate into beige (1–3; black bars) (BA) and white (4; white bars) (WA) adipocytes. Gene expression values are relative to ARP. (B) Representative blots and relative UCP1 and GLUT4 protein expression in 3T3-L1 fibroblasts that were induced to differentiate into beige (1–3; black bars) and white (4; white bars) adipocytes in relation to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (C) Lipid droplets characterization by triacyclglycerols staining with Oil Red O expression in 3T3-L1 fibroblasts that were induced to differentiate into beige and white adipocytes. Scale bar = 20 µm. Data represent mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.