Skip to main content
. 2020 Jan 17;21(2):610. doi: 10.3390/ijms21020610

Figure 2.

Figure 2

AQP7 and AQP9 differential expression in beige and white adipocytes. (A) Relative expression of several beige adipocyte markers (CD137, TBX15, and TBX1) and (B) aquaporins (AQP5, AQP3, AQP7, and AQP9) in 3T3-L1 fibroblasts induced to differentiate into beige (BA; black bars) and white (WA; white bars) adipocytes. Gene expression values are relative to ARP and to Eef2. (C) Representative blots and relative AQP9 protein expression in 3T3-L1 fibroblasts that were induced to differentiate into beige (BA; black bars) and white (WA; white bars) adipocytes in relation to GAPDH. A single band of 33 kDa was detected in cultured cells. (D) Antibodies validation against AQP5, AQP3, AQP7, and AQP9 in murine tissues: Heart, BAT, testis, pancreas, and WAT. Data represent mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01; *** p < 0.001, beige vs. white adipocytes.