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. 2020 Jan 17;21(2):610. doi: 10.3390/ijms21020610

Figure 3.

Figure 3

Beige phenotype achievement along differentiation. (A) Relative expression of UCP1, (B) TIMM44, (C) NRG4, TBX15, CD137, (D) AQP3, AQP7, and AQP9 and (E) AQP5, in 3T3-L1 fibroblasts induced to differentiate into beige (BA; black bars) and white (WA; white bars) adipocytes at day 0, 4, and 7 of differentiation. Gene expression values are relative to ARP and Eef2 and to day 0. (F) Triacylglycerols accumulation in 3T3-L1 fibroblasts induced to differentiate into beige (BA; black bars) and white (WA; white bars) adipocytes at day 0, 4, and 7 of differentiation. Data represent mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, differentiation time point vs. previous time point.