Figure 3.

Significantly elevated mitochondrial superoxide levels in normal and diseased PTEC during high-level oxidative stress. (A) Fold changes (relative to 0 mM H₂O₂ cells) in mitochondrial superoxide levels (% MitoSOX⁺ cells) in normal and diseased PTEC cultured under low-level (0.4 mM H₂O₂) and high-level (0.8 mM H₂O₂) oxidative stress conditions. The dashed line represents a fold change of 1. Symbols represent individual donor PTEC; n = 5 donor PTEC per group. Horizontal bars represent medians, with interquartile range also presented. * p < 0.05 vs 0 mM H₂O₂, Friedman test with Dunn’s post-test. (B) Representative donor histograms of MitoSOX staining (black unfilled) in normal and diseased PTEC cultured in the absence (0mM) and presence (0.8 mM) of H₂O₂ compared with unstained control (grey filled). Mitochondrial superoxide levels (% MitoSOX⁺ cells) are presented for each histogram, with fold change (FC) values relative to 0 mM H₂O₂ cells also shown.