Figure 5.

Diseased PTEC maintain mitochondrial function under high-level oxidative stress conditions. (A,B) Fold changes (relative to 0 mM H₂O₂ cells) in mitochondrial mass (JC-1 green fluorescence) (A) and mitochondrial membrane potential (MMP) (JC-1 red fluorescence) (B) in normal and diseased PTEC cultured under low-level (0.4 mM H₂O₂) and high-level (0.8 mM H₂O₂) oxidative stress conditions. The dashed line represents a fold change of 1. Symbols represent individual donor PTEC; n = 5 donor PTEC per group. Horizontal bars represent medians, with interquartile range also presented. * p < 0.05 vs 0 mM H₂O₂, Friedman test with Dunn’s post-test; # p < 0.05, Mann–Whitney test. (C) Representative donor JC-1 dot plots of normal and diseased PTEC cultured in the absence (0 mM) and presence (0.8 mM) of H₂O₂. JC-1 red delta median fluorescence intensity (MFI) values (MFI test—MFI unstained control) are presented for each dot plot, with fold change (FC) values relative to 0 mM H₂O₂ cells also shown.