Figure 2.

Cytonemes developed on the surface of human neutrophils in the presence of metabolic inhibitors. Scanning electron microscopy images of neutrophils that were adhered to fibronectin-coated substrata during 20 min at 37 °C: (A) under control conditions; (B) in the medium where Na⁺ ions were substituted by K⁺ ions; (C) in the presence of 1 mM of iodoacetic acid (IAA), inhibitor of glycolysis; (D) in the presence of 100 μM 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (Cl-NBD), vacuolar-ATPase inhibitor. The photographs shown in this figure are similar to the photographs published in our previous article [28].