Figure 1.

Schematic representation of the experimental protocols and the measurement of body weight. (A) The control group was fed normal chow, and two of the thirteen mice were sacrificed after 4, 6, and 12 weeks. The cuprizone group was fed a 0.2% cuprizone (CPZ) diet, and two of the thirteen mice were sacrificed after 4 and 6 weeks, respectively. The CPZ group was fed the CPZ-containing diet for 6 weeks and then allowed to recover for 6 weeks on normal chow without CPZ. Behavior assessments were conducted in the two groups at 0, 6, 8, 10, and 12 weeks during the experimental period, and two of the nine mice were sacrificed at 6 weeks after CPZ withdrawal, respectively. (B) The body weight of the mice was measured weekly throughout the experimental period. The final data was made with the results of the total individuals minus the number of sacrificed mice. N = 7 for each group, and * p < 0.05, ** p < 0.01.