Figure 5.

Induction of demyelination in cuprizone-fed mice. (A) Luxol fast blue (LFB)/Cresyl Echt Violet staining in coronal slices of the cuprizone-fed and control groups in the corpus callosum. Myelinated fibers are shown in blue, and Nissil substance and nerve cells in violet. Lithium carbonate indicates differentiation, and Cresyl Echt Violet is a counterstain. Scale bar: 50 μm. (C) Myelin basic protein (MBP) immunostaining in coronal slices of the cuprizone-fed and control groups in the corpus callosum. Control mice display a normally myelinated corpus callosum. Maximal demyelination is observed after 4 weeks on a cuprizone diet, and the recovery of demyelination is weakly observed after 6 weeks on a cuprizone diet. Scale bar: 50 μm. For each staining, three sections per animal were used and a total of two mice were tested each group. The staining was done at least twice each. (B,D) The integral optical density of LFB or MBP revealed using i-Solution software.