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. 2020 Jan 19;21(2):653. doi: 10.3390/ijms21020653

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Generation of TERT^+/− tumor cells by the exon removal strategy using sg4 and sg5. (A) Efficiencies of E4 removal by using both sg4 and sg5. (B) Representative genotyping results of a TERT^+/− Hela cell clone. M: molecule weight markers. (C) Telomerase activity in WT and TERT^+/− Hela cells at 1×, 10× and 100× dilutions determined by the TRAP assay. N: heat inactivated negative control. M: molecule weight markers. (D) Relative telomere content T/S ratio in WT and TERT^+/− Hela cells. ** p < 0.01.