Figure 2.

Stripping of surface IgE antibodies bound to FcεRI of basophils by acetic acid buffer (pH 4). Basophils were incubated on ice for 5 min either with phosphate buffered saline (PBS) or ice-cold acetic acid buffer pH 4 (0.05 M acetate, 0.085 M NaCl, 0.01 M EDTA and 0.03% human serum albumin) (AA pH 4). Cells were then washed and proceeded with phenotype analyses by flow cytometry. (A) The viability of cells immediately following AA pH 4 treatment as analyzed by fixable viable dye staining. (B) Efficacy of stripping of basophil surface-bound IgE by AA pH 4 (right panel) as analyzed by surface staining of IgE and analyses by flow cytometry. Representative data from four donors are presented.