Figure 2.

Cleavage analysis of a panel of 69 recombinant human cytokines and chemokines. Four micrograms of the various recombinant cytokines and chemokines in buffer solution were divided to cover the following experiments: one as a negative control (C) where no enzyme was added, one was cleaved with hNE and one with hPR3. The cleavage was performed at 37 °C for 2.5 h. The samples were separated on 4–12% SDS-PAGE gels under reducing conditions. Size markers are found at the left side of each gel. The gels were stained in colloidal Coomassie blue solution. Due to the relatively high concentration of hPR-3 it is visible on the gels and there marked by an arrow. The entire experiment with all the 69 cytokines and chemokines was repeated once with a new batch of cytokines and chemokines and the result was almost identical to what is shown in Figure 2.