Figure 3.

Cleavage analysis of a smaller panel of recombinant human cytokines and chemokines by a combination of three neutrophil proteases. Three micrograms of the various recombinant cytokines and chemokines in buffer solution were divided into two separate tubes. One was kept as negative control (C) where no enzyme was added, one was cleaved with a combination of three neutrophil proteases hNE, hPR3 and hCG. The cleavage was performed at 37 °C for 2.5 h. The samples were separated on 4–12% SDS-PAGE gels under reducing conditions. Size markers are found at the left side of each gel. The gels were stained in colloidal Coomassie blue solution. This analysis was performed only once.