Table 1.
Summary of the results from the cleavage analysis of cytokines and chemokines by hNE and hPR-3 and the mix of the three neutrophil proteases hNE, hPR-3 and hCG. The analysis is based on the SDS-PAGE analysis in Figure 1 and Figure 2. (-) no cleavage activity was observed. (+) showed minor activity, (++) and (+++) partial cleavage. (++++) complete or almost complete cleaved by the enzyme. For more easy identification brackets, ¨ ¨, are inserted for the cytokines and chemokines that appear to be relatively resistant to cleavage. The results for the human mast cell chymase (HC) and human cathepsin G (hCG) are all from a previous publication [10].
| Cytokine | HC | hCG | hNE | hPR3 | Mix | Cytokine | hNE | hPR3 | Mix |
|---|---|---|---|---|---|---|---|---|---|
| IL-4 | - | - | - | ++++ | M-CSF | ¨+¨ | ¨+¨ | ||
| IL-5 | ¨-¨ | ¨-¨ | ¨-¨ | ¨+¨ | VEGF-121 | ++ | + | ||
| IL-10 | - | - | + | +++ | IL-16 | ++++ | ++++ | ||
| TSLP | + | - | ++++ | ++++ | FGF-9 | ++++ | ++++ | ++++ | |
| IL-13 | + | - | +++ | ++++ | IL-1α | ¨-¨ | ¨-¨ | ||
| SCF | - | ++++ | ++++ | ++++ | IL-1RA | ¨-¨ | ¨+¨ | ¨+¨ | |
| IL-3 | + | ++++ | ++++ | ++++ | IGF-1 | ++++ | ++++ | ++++ | |
| GM-CSF | - | + | + | +++ | IGF-2 | ++++ | ++++ | ++++ | |
| IL-9 | - | - | sIL-6R | + | +++ | +++ | |||
| G-CSF | - | - | ++++ | ++++ | CNTF | ++++ | ++++ | ++++ | |
| IL-2 | - | + | ++ | +++ | ++++ | HGF | ++ | +++ | |
| IFN-γ | + | + | + | ++++ | ++++ | FGF-19 | ++++ | ++++ | ++++ |
| IL-15 | ++++ | ++++ | ++++ | ++++ | ++++ | BMP-14 | ¨-¨ | ¨-¨ | ¨+¨ |
| IL-12 | - | - | - | ++ | GAL-7H | +++ | ++++ | ||
| IL-20 | - | - | ++ | +++ | Neuregulin-1a | ++++ | + | ++ | |
| IL-1β | - | - | + | ++ | Neuregulin-1b | ++++ | ++++ | ++++ | |
| TNF-α | ¨-¨ | ¨+¨ | ¨+¨ | ¨+¨ | ¨+¨ | PF4V1 | ++++ | ++++ | ++++ |
| IL-6 | ++ | ++++ | +++ | ++++ | ++++ | TARC | + | ++++ | ++++ |
| IL-11 | + | + | + | ++++ | TRAIL | ++ | +++ | +++ | |
| LIF | + | + | ++ | ++++ | BMP-2 | ¨-¨ | ¨-¨ | ||
| IL-7 | - | +++ | ++++ | ++++ | TGF-β3 | ++++ | - | ||
| IL-21 | - | ++ | +++ | ++++ | GROα | - | ++++ | ++++ | |
| IL-24 | - | + | |||||||
| IL-31 | - | ++++ | ++++ | ++++ | |||||
| IL-19 | - | - | ++ | ++++ | ++++ | ||||
| IL-17A | - | ++ | ++++ | ++++ | ++++ | ||||
| IL-17F | - | - | +++ | ++ | |||||
| IL-22 | - | + | ++ | ++++ | |||||
| sCD40L | - | + | + | ++ | ++ | ||||
| BAFF | + | + | ++ | ++ | |||||
| VEGF-A | - | +++ | ++ | ++++ | |||||
| BMP-7 | - | + | |||||||
| PDGF-A | - | - | +++ | ++ | |||||
| PDGF-B | ¨-¨ | ¨-¨ | ¨+¨ | ¨-¨ | |||||
| IL-33 | ++++ | ++++ | ++++ | ++++ | ++++ | ||||
| CTGF | + | +++ | ++++ | ++++ | ++++ | ||||
| FGF-1 | + | ++++ | ++++ | ++++ | ++++ | ||||
| FGF-2 | + | + | ++ | + | ++ | ||||
| flt3L | ++ | + | ++++ | ++++ | |||||
| IL-18 | +++ | ++++ | + | +++ | ++++ | ||||
| EGF | ¨-¨ | ¨-¨ | ¨-¨ | ¨-¨ | ¨-¨ | ||||
| IL-8 | ¨-¨ | ¨-¨ | ¨-¨ | ¨++¨ | |||||
| IP-10 | - | - | - | ++++ | ++++ | ||||
| MCP-1 | ¨-¨ | ¨-¨ | + | ¨-¨ | ¨-¨ | ||||
| MCP-2 | - | - | - | ++ | |||||
| MCP-3 | - | - | + | ++++ | |||||
| RANTES | ¨-¨ | ¨-¨ | ¨-¨ | ¨+¨ | ¨+¨ | ||||
| SDF-1α | - | - | ++ | ++++ | |||||
| SDF-1β | - | + | ++ | ++++ | |||||
| MIP-1α | - | - | - | ++++ | ++++ |