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Analysis of the CL2 domain as a potential CaM-binding site. The CaM sites were identified by a program that predicts them on the scale 0–9. In all but human-Cx40 (Cx40h), the CL2 domain contains a CaM site. CaM-binding to the CL2 domains of Cx32, Cx35, Cx43, Cx44, Cx45, Cx50 and Cx57 has been experimentally demonstrated. The lack of a binding site in Cx40h may be due to the absence of two valine residues (arrows) present in Cx40r.
