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. 2020 Jan 15;21(2):553. doi: 10.3390/ijms21020553

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Molecular profile of phage infection of the four genera of Erwinia phages in this study. (a) Infection of E. amylovora Ea6-4 by phage ΦEa21-4 at an multiplicity of infection (MOI) of 0.45. (b) Infection of E. amylovora EaD7 by phage ΦEa46-1-A1 at an MOI of 0.625. (c) Infection of E. amylovora Ea17-1-1 by phage ΦEa9-2 at an MOI of 0.015. (d) Infection of E. amylovora Ea273 by phage ΦEa9-2 at an MOI of 0.015. (e) Infection of P. agglomerans Pa39-7 by phage ΦEa35-70 at an MOI of 0.03. (f) Infection of P. agglomerans Pa39-7 by phage ΦEa35-70 at an MOI of 0.7. The symbols represent the mean of data produced in triplicate, the line is a local regression model of the data (LOESS), and the shaded area around the line represents a 95% confidence interval. MOIs were calculated using the qPCR data at t₀. Here, (blue diamond) = total number of phage genomes; (green circle) = total number of phage genomes in the supernatant; (purple triangle = total number of virions; (red square = total number of virions in the supernatant.

Inline graphic — Molecular profile of phage infection of the four genera of Erwinia phages in this study. (a) Infection of E. amylovora Ea6-4 by phage ΦEa21-4 at an multiplicity of infection (MOI) of 0.45. (b) Infection of E. amylovora EaD7 by phage ΦEa46-1-A1 at an MOI of 0.625. (c) Infection of E. amylovora Ea17-1-1 by phage ΦEa9-2 at an MOI of 0.015. (d) Infection of E. amylovora Ea273 by phage ΦEa9-2 at an MOI of 0.015. (e) Infection of P. agglomerans Pa39-7 by phage ΦEa35-70 at an MOI of 0.03. (f) Infection of P. agglomerans Pa39-7 by phage ΦEa35-70 at an MOI of 0.7. The symbols represent the mean of data produced in triplicate, the line is a local regression model of the data (LOESS), and the shaded area around the line represents a 95% confidence interval. MOIs were calculated using the qPCR data at t₀. Here, (blue diamond) = total number of phage genomes; (green circle) = total number of phage genomes in the supernatant; (purple triangle = total number of virions; (red square = total number of virions in the supernatant.