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. 2020 Jan 11;21(2):473. doi: 10.3390/ijms21020473

Figure 1.

Figure 1

Dose-response curve of the LY294002 inhibitor over FoxO3a. The cells were incubated to a concentration of LY294002 inhibitor (0.0, 0.5, 1, 2, 5, 10, 25, 50 µM) for 24 h. Cells were cultured for 24 h in F12K medium supplemented with 10% of fetal bovine serum (FBS); Control: cells unexposed; DMSO: cells exposed only to DMSO (vehicle for LY294002). (A) Representative image of western blot of pFoxO3Ser253, FoxO3, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as housekeeping control and (B) data from a duplicated assay.