Fig. 6.

TGFBR2 overexpression induces ESCC cell cycle arrest but not cell apoptosis. a Western blot analysis of wild-type (WT) cells and lentiviral mediating the overexpression of TGFBR2 (OE) cells in KYSE-150 and KYSE-30 cell lines. b The morphology of WT and OE cells in colony formation assay. c The expression level of phospho-SMAD2 and SMAD2 in WT and OE cells. d Cell cycle distribution in WT and OE cells. Graphic representation of results from cell cycle analysis in WT and OE cells. e Annexin V staining of parental WT and KO cells to detect apoptosis using flow cytometry. Graphic representation of the percentage of apoptotic cells in parental versus OE cells. f Annexin V staining of KYSE-150 cells exposed to increasing concentrations of 5-Aza-2′-deoxycytidine detected with flow cytometry. Graphic representation of the percentage of apoptotic cells with increasing 5-Aza-2′-deoxycytidine concentration