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. 2020 Feb 11;12:26. doi: 10.1186/s13148-020-0817-8

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Radiation-induced morphological changes with increasing number of radiation fractions of 2 Gy. a Bright-field enlarged cells. b Percent of viable cells and cell diameter. c Immunocytochemistry showing cellular morphology with α-tubulin-stained cytoskeleton (orange), DNA-synthesizing cells by EdU incorporation (green) and nuclear staining with DAPI (blue). d Single-cell seeding after irradiation shows reduced clonogenic capacity after 3 fractions compared to unirradiated and 15 + 3 irradiated cells. e The frequency and staining pattern of GFAP varied between treatment groups