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. 2020 Feb 11;13:16. doi: 10.1186/s13048-020-00618-7

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© The Author(s) 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Flow of data for integrative analysis with mRNAs and miRNAs. DEG and DEmiR were selected from microarray data (paired t-test, p-value < 0.05), and targets of DEmiR were predicted with TargetScan. At first four groups of over- and under-expressed genes/target genes were analyzed independently to identify the activated or suppressed functions of SFCs. Thereafter, integrative analysis of DEG and DEmiR was performed using the MAGIA² webtool. DEG: differentially expressed gene, DEmiR: differentially expressed miRNA, SFC: spheroid-forming cell