Fig. 2.

Integrative network composed of TFs, miRNAs, and target genes. Genes of known function were grouped using dotted lines. Transcription-related genes were most common, while genes of cell cycle and cell adhesion were suppressed in SFCs. EGR1, MYC, and miR-130a-3p were the main regulators to remain as a quiescent phenotype of stem cell. Rectangle: TF, ellipse: gene, diamond: miRNA, colors indicate fold change of the expression in spheroid-forming cells relative to parental cells (red: ≥2, yellow: 2 > to > 1, green: 1 > to ≥0.5, blue: > 0.5), SFC: spheroid-forming cell, TF: transcription factor