Fig. 8.

Silencing of TANCR in HEK293T cells and Jurkat cells. a qRT-PCR results of TANCR expression in TANCR knockdown HEK293T cells. b qRT-PCR results of TRAIL mRNA expression in TANCR knockdown HEK293T cells. c Western blot of TRAIL protein in TANCR knockdown HEK293T cells. d Expression of Cas9 in Jurkat cells. Two vector CRISPR/Cas 9 system was used in this study, the first vector contains cas9 and GFP and the second vector contains gRNA. e RT-PCR results of TANCR expression in TANCR knock out Jurkat cells. f qRT-PCR results of TRAIL mRNA expression in TANCR knock out Jurkat cells. g Agarose gel electrophoresis of qRT-PCT results. h Western blot of TRAIL protein in TANCR knock out Jurkat cells. qRT-PCR and western blot were normalized by GAPDH. Graphs show the mean values ± SEM and data obtained from three independent experiments. p value is calculated by Student’s t-test