Fig. 9.

Silencing of TANCR in primary γδ T cells. a Cas9 expression in primary γδ T cells. b Expression of TANCR in TANCR KO primary γδ T cells, a guide RNA mixture (containing guide RNA 1 and guide RNA 2 used in Jurkat cells) was transduced into primary γδ T cells. c Expression of TRAIL mRNA in TANCR KO primary γδ T cells. d Agarose gel electrophoresis of qRT-PCR results. e Western blot of TRAIL in TANCR KO primary γδ T cells. qRT-PCR and western blot were normalized by GAPDH. Data are represented as means ± SEM from three individual experiments. p value is calculated by Student’s t-test