Table 1.

Acute toxicity of benzalkonium chloride in mice after treatment via oral administration or intratracheal instillation

Route	Dose (mg/kg)	Exposed duration (d)
		1	2	3	4	5	6	7	14
Oral administration	400	6	6	6	6	6	6	6	6
	300	5	5	5	6	6	6	6	6
	200	2	4	4	4	4	4	4	4
	150	0	0	2	3	3	3	3	3
	100	0	0	0	0	0	0	0	0
	0	0	0	0	0	0	0	0	0
Intratracheal instillation	30	6	6	6	6	6	6	6	6
	20	5	5	5	5	5	5	5	5
	10	4	4	4	4	4	4	4	4
	5	4	4	4	4	4	4	4	4
	2.5	2	2	2	2	2	2	2	2
	1.25	0	0	0	0	0	0	0	0
	0	0	0	0	0	0	0	0	0

The accumulated number of dead mice were represented after treatment of benzalkonium chloride at the designated observation period. Total 6 mice were held in a group and treated with benzalkonium chloride.